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KMID : 0545120110210101053
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 10 p.1053 ~ p.1056
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Production of a Platelet Aggregation Inhibitor, Salmosin, by High Cell Density Fermentation of Recombinant Escherichia coli
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Seo Myung-Ji
Choi Hak-Jong
Chung Kwang-Hoe
Pyun Yu-Ryang
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Abstract
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Optimal conditions for a high cell density fermentation were investigated in a recombinant Escherichia coli producing salmosin, a platelet aggregation inhibitor. The optimized carbon and nitrogen sources were glycerol 10 g/l, yeast extract 30 g/l, and bacto-tryptone 10 g/l, yielding the dry cell weight (DCW) of 10.61 g/l in a 500 ml flask culture. The late-stage induction with 1% L-arabinose in a 5 l jar fermentor showed the highest DCW of 65.70 g/l after 27 h of the fed-batch fermentation. Around 2,200 mg/l of the protein was expressed as an inclusion body that was then refolded to obtain the active salmosin of 96 mg/l. We also confirmed the inhibitory activity against platelet aggregation of the active salmosin from the high cell density fermentation.
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KEYWORD
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High cell density fermentation, platelet aggregation inhibitor, recombinant Escherichia coli, salmosin
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